Description

Edvotek Lighting Up Life - Expression of GFP in C. elegans Kit contains instructions, C. elegans, E. coli OP50 cells, S-Buffer, Ready-Pour NGM Agar, NGM medium salts, UltraSpec-Agarose powder, electrophoresis buffer, 10x gel loading solution, InstaStain ethidium bromide, FlashBlue liquid stain, tubes, pipets, wax beads, petri dishes, counting chamber, microscope slides, sterile loops. It includes sufficient materials for 10 lab groups. Scientists use engineered genes called transgenes to directly manipulate an organism's genome for producing a phenotype. Kit helps students to explore genetic engineering. Students can use fluorescent microscopy and PCR for analyzing C. elegans that have been engineered to express the green fluorescent protein. Students require covered box, fluorescent and dissecting microscopes, timers, waterbath, incubator, thermal cycler or three water baths, UV transilluminator or white light visualization system, electrophoresis apparatus, balance, microcentrifuge, automatic micropipets with tips, microwave or hot plate, pipet pump, flasks or beakers, hot gloves, distilled or deionized water, power supply, ice buckets and ice to conduct the experiment.